Human health risk increases with higher exposure to free radicals. An imbalance between the number of free radicals and antioxidants in the body results in oxidative stress. Oxidative stress triggers a variety of diseases, including cardiovascular disease. Betamelor contains anthocyanins, flavonoids and isoflavones, it is expected to reduce oxidative stress. The purpose of this study was to analyze the potential of betamelor (black rice, red beans, and moringa leaves) on cholesterol levels in rattus norvegicus rats induced by a high-fat diet. This is an experimental study with pre and post control group design. A total of 30 female of rattus norvegicus strains aged 5-6 months with a weight of 150-200 g were induced orally using a high-fat diet about two ccs/day. Experimental animals were divided into five groups, namely standard feed (K0), standard feed with 2cc/day of simvastatin (K1), 20% betamelor-based feeding (K2), 50% betamelor-based feeding (K3), and 80% betamelor-based feeding (K4). Betamelor was intervened about 5% of bodyweight for 14 days. Blood sampling and analysis were carried out twice, at week 0 (before the intervention) and week 2 (after the intervention). Blood was drawn directly from the eye vein (ocular artery) ± 2 ml using microhaematocrit capillary tubes after anesthetized with 10 mg/kg body weight of ketamine. Cholesterol data were analyzed by Analysis of Variance (ANOVA) at a 95% confidence level. If the results of the analysis show a significant effect on the response variable, then a Tukey test will be conducted. Data processing and analysis were performed using Microsoft Excel and SPSS for Windows version 22.0. Result: There were significant differences (p <0.05) in the K2, K3, and K4 groups, with total blood cholesterol levels of 10.1 mg/dl, 18.0 mg/dl, and 22.02 mg/dl, respectively, compared to the control group (K0). There were significant differences found in the K2 and K3 groups compared to the positive control group (K1). Comparison