The purpose of this research was to evaluate effect of encapsulated coconut shell liquid smoke as a replacement for antibiotics in broiler feed on active substances and intestinal microflora. The materials used in this research were 225 Day Old Chick from Cobb strains, which were allocated into 5 treatments and 5 replications. The treatments used in this research were T0 (basal feed) as a control treatment, T0 + (basal feed + antibiotic) as a positive control treatment, T1 (basal feed + 0.5% encapsulated coconut shell liquid smoke), T2 (basal feed +1 encapsulated coconut shell liquid smoke), T3 (basal feed + 1.5% encapsulated coconut shell liquid smoke). The variables measured were active substance contents and intestinal microflora population including Lactic Acid Bacteria (LAB), Salmonella sp, and Escherichia coli. The microflora data were analyzed by using One Way Analysis of Variance and if there is a significant difference, the different test was performed with Duncan's multiple range tests. The results showed that a slight decrease in the total phenol was found, but total acid and pH of the encapsulated coconut shell liquid smoke markedly changed. In addition, encapsulated coconut shell liquid smoke at 1.5% in broiler feed significantly (P <0.01) increased LAB and decreased Salmonella sp. The conclusion of this research was the addition of encapsulated coconut shell liquid smoke 1.5% could be considered to replace the use of antibiotics in broiler feed.