This study utilized descriptive survey design in assessing the awareness and implementation of anti-plagiarism tools by post-graduate students in the University of Port Harcourt, Rivers State, Nigeria. Two research questions and two null hypotheses guided the study. A sample size of 393 comprising of 278 Masters’ students and 115 Ph.D. students drawn from the population of 557 was used for the study. The instrument for data collection was titled “Awareness and Implementation of Anti-Plagiarism Tools Checklist (AIAPC), and was developed by the researchers. Both face and content validities of the instrument were determined. Mean (x̄) and standard deviation (±) were used to answer the research questions, while independent t-test was used to test the hypotheses at 0.05 alpha level. The results revealed that the Ph.D. students are more aware of the various anti-plagiarism tools than the Masters students based on the weighted mean score (Ph.D. 2.38 ≥ Masters 1.65). The results also revealed that both the Ph.D. and the Masters students have poor implementation of the various anti-plagiarism tools. This was shown in their weighted mean scores which is less than the criterion mean score of 2.50 (1.65, 2.38 ≤ 2.50). Based on the result, it was recommended among others that post-graduate students should be exposed to courses on plagiarism.