Background: The present study was aimed to determine the prevalence of cholelithiasis among a general population in Gujranwala, Punjab, Pakistan. Methods: Between August 2020 and December 2020, we conducted a cross-sectional sample of patients who presented to a private hospital in Gujranwala for abdominal ultrasound. Ultrasonography was used to evaluate the gallbladder. Cholelithiasis was characterized as the appearance or absence of gallstones. The data was analyzed using descriptive statistics. Results: The male participants were 42% and female participants 58%. The married participants were 88 percent and rest of 12% were unmarried. The highest frequency of cholelithiasis is seen among the age of 28-47 years patients. Regarding Gall bladder appearance, 76 percent of the participants have normal appearance. 6% with distended and16% showed the contracted appearance whereas in 2% cases Gall bladder was surgically absent. In 90% of the cases the sludge was absent in gall bladder and only 10% were filled with sludge. Regarding Gall bladder wall thickness 86% of the participants had normal thickening and in 12% wall was thickened. Whereas 2% cases already had surgically absent GB. The Gall bladder stones were absent in 40 % participants and 60% had stones present in. Conclusions: Incidence of cholelithiasis among patients undergoing abdominal ultrasonography in Gujranwala region was higher in females as compared to males. The married participants showed higher prevalence of GB stones. Patient age, gender and marital status significantly influenced the prevalence.