This study was conducted to assess allelic and genotypic frequencies at the selected blood protein [Albumin (Alb.), Haemoglobin (Hb.) and Transferrin (Tf)] loci in indigenous turkey populations in Southern Guinea Savannah of Nigeria. Blood samples were collected from 42 birds, 14 birds each from Benue, Kogi and Nasarawa States via purposive sampling. The blood was analysed by electrophoresis. The genetic relationship of the population was determined by using Tools for Population Genetics Analysis (TFPGA), version 1.3. Result obtained indicated that, two co-dominant alleles (A and B) which controlled three different genotypes (AA, AB and BB) were observed for Alb and Tf while only two genotypes (AA and BB) for the Hb polymorphic loci were observed. Sexual differences in gene and genotypic frequencies of Alb, Hb and Tf of male and female turkeys showed that genotype Alb AB and allele A were prevalent in the male while genotype Alb BB and Allele B were predominant in the female turkey. However, at the haemoglobin locus, genotype HbAA and allele A were prevalent in the male and female turkey, respectively. For tranferrin, genotype TfAB was most predominant in the male with equal frequencies in both alleles while genotype TfBB and allele B were most prevalent in the female. From the estimates of variability parameters in the populations under study, it can be concluded that the populations are under similar evolutionary forces and there were no appreciable differences among them. It was recommended that the result of this study be used as initial guide for defining objective of genetic improvement and developing strategies for conservation of Nigerian indigenous turkey.