EAS Publisher | A Multiplex Assay for Simultaneous Detection of Three Retroviruses in Nonhuman Primates Based on the Luminex xTAG Platform

A Multiplex Assay for Simultaneous Detection of Three Retroviruses in Nonhuman Primates Based on the Luminex xTAG Platform

DOI : https://doi.org/10.36349/easjvms.2021.v03i02.001

PDF

HTML

XML

1024 Downloads 3 Views

Nonhuman primates (NHPs) are widely used for investigating retroviral pathogenesis. Before experiments are conducted and breeding programs established, the animals must be judged negative for endogenous retroviruses. We developed a multiplex high-throughput detection method using multiplexed Luminex fluorescent microbeads and the xTAG system (MMxTAG). We tested three retroviral DNA detection assays for use in a single tube reaction by taking advantage of the Luminex xTAG platform. The assay showed a high sensitivity that was comparable to qPCR methods. The detection limits approached 1-10 fg/μL of plasmid DNA. We compared the specificity, efficiency and accuracy with ELISA assays and found our assay was superior in all regards. The MMxTAG performed well with clinical specimens and could selectively detect a single virus in the presence of the other two. This MMxTAG procedure can be applied to pathogen monitoring in animals and is present in a high throughput format that has low operating and development costs for protecting monkey contacts against these infectious diseases.

TOP EDITORS

Dr. T. Selvankumar
Chief Editor
EAS Journal of Biotechnology and Genetics

Dr. James Kay, PhD
Chief Editor
EAS Journal of Psychology and Behavioural Sciences

Dr. Rejeesh Menon
Chief Editor
EAS Journal of Medicine and Surgery

Dr. S. Jayachandran
Chief Editor
EAS Journal of Dentistry and Oral Medicine

Dr. Md. Habibur Rahman
Chief Editor
EAS Journal of Pharmacy and Pharmacology

Dr. Benard Chemwei, PhD
Chief Editor
East African Scholars Multidisciplinary Bulletin

NFI Joseph Lon
Chief Editor
EAS Journal of Humanities and Cultural Studies

Prof. Dr. Nazir Ahmad Suhail
Chief Editor
East African Scholar Journal of Engineering and Computer Sciences

Dr. Hamid Osman Hamid
Chief Editor
EAS Journals of Radiology and Imaging Technology

Dr. BOUCENNA Mounir
Chief Editor
EAS Journal of Veterinary Medical Science

OPEN ACCESS JOURNALS

Dr. Afroza Begum

Lecturer, Dept. of Pharmacology and Therapeutics, Shaheed Monsur Ali Medical College & Hospital, Uttara, Dhaka-1230, Bangladesh

BEST AUTHOR

Of The Month

TRACK YOUR ARTICLE

Enter the Manuscript Reference Number (MRN)

Get Details