Abstract: Background: Detrimental effects of homocysteine on endothelial function are well documented. Plasma Homocysteine (Hcy) levels are elevated in type 2 diabetic patients as well as in pre-diabetic individuals with insulin resistance. In such individuals, plasma Hcy concentration is influenced by the insulin concentration and anti-diabetic therapy such as metformin, glitazones or insulin that can alter the plasma plasma homocysteine. Objective: To find out the Clinical & Demographic Profile with Serum Homocysteine Level among Type II Diabetic Patients and IGT Patients with Normal People. Methods: This was a cross-sectional observational study. Total 120 subjects were selected and allocated into three groups, equal number (n=40) in each group. This study was conducted in Mymensingh Medical College Hospital, Mymensingh, Bangladesh from June, 2019 to May, 2020. Results: The study showed that the mean age of the patients and their BMI were almost similar among the three groups, male female ratio was about 2.5:1. The ccorrelation analysis of different clinical & demographic profile with serum Homocysteine. The correlation between glycemic status and Serum Homocysteine levels of participants, which differ considerably, so that, Pearson correlation & Spearman correlation showed statistically significant difference. Hcy was positively associated with FBG (r=0.296, p<0.001), 2hPG (r=0.078, p=0.004). Similarly positive significant correlation was found with TC (r=7.655, p<0.001), TG (r=13.52, p=<0.05) and HDL-C (r=1.165, p<0.05). Present study showed that levels of Hcy were significantly higher in the type 2 DM group than those in normoglycemic group (19.86 μmol/L vs. 8.72 μmol/L, p<0.001). Similarly levels of Hcy were significantly higher in the IGT group than those in normoglycemic group (17.28 μmol/L vs. 8.72 μmol/l, p<0.001). The mean values of the serum Homocysteine level of Group -1 and Group – 2 showed statistical significant differences ..................