Abstract: Despite the importance of coffee to the national economy and livelihood of Ugandans, its yields remain low due to a number of constraints, with pests and diseases being paramount. The recent outbreak of dodder weed, Cuscuta spp. is threatening the coffee sub-sector, particularly in Busoga sub-region. A rapid assessment was therefore conducted in the Busoga sub-region to determine farmers’ knowledge as well as status, distribution and damage of dodder weed on coffee and other plant species. Our results showed that all the respondents had knowledge of Cuscuta spp. However, only 57.1% of them had observed the weed on coffee and only recently - between 2019 and 2020 (50%). At plot level, dodder was observed in all the surveyed districts. On average, dodder was recorded on 40% and 10.4% of the coffee gardens and trees sampled respectively. It was also observed on 33 other plant species, with highest scores being recorded on: - M. lutea (18.6%), T. peruviana (15.7%) and D. erecta (8.6%). Respondents mentioned that dodder was mostly dispersed by humans – children (40%) and herbalists (5.1%). Further, 28 alternative hosts of dodder were mentioned by the respondents, with Thevetia peruviana (64%), Mangifera indica (40%), Duranta erecta (24%) as well as Artocarpus heterophyllus and Markhamia lutea (14%) being most outstanding. Few of the respondents had knowledge of the use of dodder – witchcraft (24.3%) and herbal medicine (5.7%). Most (81%) of the respondents were managing dodder by physically removing it. This study therefore provides baseline information for developing sustainable management strategies for dodder in coffee agro-systems.