Abstract: Background: Central venous catheters (CVC) are known to be associated with risk of complications such as infection and hemorrhage thus good knowledge and practices of care are crucial in limiting these risks also nurses be supposed to integrate evidence-based practice into their care to improve patient outcomes. This study aimed to evaluate impact of mini structured education program on ICU nurses knowledge and practice regarding prevention of central venous line associated blood stream infection. Methodology: This is an interventional pre –posttest study conducted in Alshaab Teaching Hospital, full coverage sampling method was used incorporated 32 ICU nurses working in ICU and met selection criteria, Information and skill were collected by researchers from ICU nurse’s using standardized self-administer questionnaire and direct observation check list . Mini structured education program was applied and post-test with final assessment were conducted after 2 weeks using same questionnaire and check list then the collected data pre and post analyzed analyzed and interpreted using both descriptive and inferential statistics based on the objective and hypothesis of the study. A two-tailed p value at 0.05 was taken as the level of significance using SPSS version 20. Results: Among 32 nurses included; mean age of participant was 25 years, almost of participants were female 87.5% while(29) 90,6% had BSc level of qualification and84,4% more than 1year duration of work. The overall mean of pre-test knowledge scores regarding prevention of central venous line associated blood stream infection was found to be 5.8 with statically significant difference (p=0.000<0.05) and in post-test found to be increased to 8,2with statically significant difference (p=0.000<0.05) . Regarding practice the overall mean of pre-test scores toward care of CVL in order to prevent central venous line associated blood stream infection was found to be 11.8with statically ...............