Abstract: Gum Arabic (GA) is known exudative polysaccharide from gum acacia trees and has been used as an antioxidant, to protect the liver, kidney and heart against different toxicants. The current study was undertaken to estimate the effect of GA on the inflammatory factors, hepatocytes apoptosis and antioxidant status, of rats suffering from hepatotoxicity caused by Carbon Tetrachloride (CCl4). Four experimental groups (n=8) were established. Group A used as a control and received basal diets only. Groups (B & D) received basal diets containing GA (5%). Groups (C & D) were injected with CCl4, to induce hepatotoxicity in rats twice a week, for 3 weeks. GA is found to improve the amount of total protein (TP) in the serum, and the activities of glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and superoxide dismutase (SOD). In addition, hepatic expression of glutathione peroxidase1 (GPX1) and superoxide dismutase1 (SOD1), were also increased, while effectively decreased the tissue levels of lipid peroxidation (MDA), serum amount of alanine amino transferase (ALT) and aspartate amino transferase (AST). Moreover, GA down-regulate the expression levels of hepatic caspase3, IL-β1, IL-6 and TNF-α genes that were increasing in CCl4 only treated group. Histopathological evaluation of the liver in rats treated only by CCl4 revealed cellular necrosis, fatty degeneration with inflammatory changes, however, GA revealed a noticeable amelioration of the severity of these changes. Dietary administration of GA has a beneficial result on the hepatic apoptosis, oxidative stress and inflammatory response in experimentally- induced hepatotoxicity in rats.