Abstract: This study was carried out in lath house belong to Department /Horticulture and Landscape design /college of Agriculture and Forestry / University of Mosul for the period from (1 July 2019) to (1 December 2019), to determine the effect of foliar application of proline at (zero, 25, 50) mg.l-1 as well as treatment with Optimus plus fertilizer which was produce according to nano technology consist of (5% Nitrogen, 30% Amino acid, 3% Organic nitrogen) at (zero, 1) ml.l-1 sprayed on the vegetative growth and between sprinkle and the next 20 days, the last factor include three irrigation levels of watering at (100%, 50%, 25%) of filed capacity in growth of pelargonium graveolens L.. The experiment were analyzed statistically by using Factorial with in Split-Plot Design in randomized Complete Block Design with three replication and five plant per treatment, was used. Duncan test used to compare between means at 0.05 probability level. The irrigation level 25% of F.C caused significant decrease in the most of studded parameters. The difference in spraying with the amino acid proline caused a significant increase in the parameters of: number of branches, length of the longest root, proline content and volatile oil ratio. But spraying by nano fertilizer Optimus plus cause significantly increased the all growth, chemical and anatomic parameters.