Milk Ring Test (MRT) was conducted to determine the seroprevalence of bovine brucellosis in dairy cows in Bahri North locality, Sudan, to evaluate the associated risk factors of brucellosis as well as to estimate the qualitative risk of brucellosis to public health from consuming raw milk contaminated with Brucella. The Seroprevalence of brucellosis in dairy cows screened was found to be 33.3% using MRT. Chi-square test showed significant association between brucellosis and risk factors such as breed (P-value.030), rearing system (P-value.008), sharing of bull- for fertilization with other farms (P-value.008), and testing of new animals before introducing them into the farm (P-value.030). All results comparing the association between Chi square and the rearing system were found to be significant in the Logistic Regression (P-value.041).Value chain using the OIE frame work for risk analysis with some adjustments as to fit with an assessment for an endemic disease, together with value chain analysis designed by FAO for disease management were used to qualitatively assess the risk for the spread of brucellosis amid dairy cows and hence milk produced. The release pathway of brucellosis in value chain represents the probability of spread of Brucella into the farm and prodsucing contaminated milk , this was found to be high which means the risky event is likely to occur this year or in frequent intervals. In this study the exposure risk represents probability of producing and marketing of milk that contaminated with Brucella, which was assessed to be medium, which means the risky event is likely to occur more than once in the next three years as explained by the DEFRA model. The overall risk estimation for brucellosis was found to be high, which means the risky event is likely to occur among farms workers, and consumers this year or at frequent intervals.