Background and Objective: Growth is considered outcome of interactions among several factors, where thyroid hormones, cortisol, aldosterone and testosterone plays a key role in coordination of these factors and the information of these hormones in relation to age, growth and live body weight in buffalo calves is not adequate. The objective of the study was to determine the changes in blood biochemical components and hormonal levels in relation to the change in each of age, live body weight and body weight gain of Egyptian male buffalo’s calves from birthing to puberty age. Materials and Methods: The present study was carried out on 10 male buffalo calves from at birthing until 24 months of age. The animals were weighed at birth, 3, 6, 12, 18 and 24 months and daily body weight gain (DBWG) was calculated. During weighing the calves, 0ne blood sample from the Jugular vein of each calf was withdrawn with anticoagulant and plasma was separated to estimate thyroxin (T4), triiodothyronine (T3), cortisol, testosterone and aldosterone hormonal level as well as glucose and protein fractions. Results and Discussion: The highest level of T4 was at birthing (120.2 nmol/l) and decreased to 95.9 nmol/l at weaning. The highest level of T3 was found at birthing (8.4nmo/l) and decreased to 5.9 nmol/l at weaning age. The lowest cortisol level was at birthing (7.3 ng/dl) while the highest cortisol value was at weaning (23.2 ng/dl) and after 24 months of age (22.6 ng/dl). The highest level of aldosterone was 7.6 ng/ml at birth and 7.3 ng/ml at weaning. Testosterone level increased progressively with increase the age of buffalo calves. T4, T3 and aldosterone hormones have negative significant correlations with each of age, LBW and DBG of buffalo calves. While testosterone level has a highly positive correlation with each of age, LBW and DBG of buffalo calve. Glucose and globulin levels have a negative significant correlation with each of age, LBW and DBG of buffalo calves. Conclusion: