Abstract: The study of gene expression was conducted by 8 Acinetobacter baumannii isolates. This study focused on effect of the Lactobacillus casei and Lactobacillus plantarum on Gene Expression of OmpA and EpsA Genes in A. baumannii isolates. The primers for A.baumannii targeting OmpA and EpsA genes are highly specific for expressing. Gene expression of OmpA and EpsA genes was quantified through Real- time PCR in A.baumannii isolates were before and after the treatment with the Lactobacillus casei and Lactobacillus plantarum, the isolates was selected that appeared highly Ct (cycle threshold) value. Ct value was determined as the point (cycle) at which the amplification plot crossed the threshold line. Eight isolates (AS-1, AS-2, AS-4, AS-5, AS-6, AS-7, AS-8 and AS-10) participated in their gene expression OmpA and EpsA genes. The qRT-PCR method has been used to examine the expression levels of the OmpA gene in Acinetobacter baumannii Isolates under the effect Lactobacillus casei and Lactobacillus plantarum. The results showed that the Lactobacillus casei and Lactobacillus plantarum increased the transcript levels of the tested gene in A. baumannii isolates with Lactobacillus casei and Lactobacillus plantarum. The results showed of (P1-P8) that OmpA gene gave a highly expression of different degrees by amplification of the gene. The Ct value of Omp A gene in the present study show the pattern of the amplification of the gene were (19.0, 23.49, 19.16, 19.14, 19.79, 20.03, 20.1 and 20.2) and folding (2.57, 0.23, 3.17, 2.59, 9.68, 5.73, 9.51 and 16.42). while (C1-C8) that OmpA gene expression recording Ct value (21.85, 21.37, 22.27, 21.83, 20.75, 20.25, 19.40 and18.59) and (1.03, 1.91,4.26,1.17,15.89,8.35,4.15 and 6.55) of folding compared to the control. The results showed of (P1-P8) that eps A gene gave a decrease expression of different degrees by amplification of the gene. The Ct value of eps A gene in the.....................