Abstract: A total of nine soil samples were collected at the depths of 0-10, 10-20 cm and 20-30cm respectively from oil polluted soils of Awoye and Osustech farm (Control). The soil samples were analyzed for physico-chemical and bacteriological properties that reflect soil nutrient content and fertility status in the laboratory using standard methods. The results of microbial flora in areas with and without oil spill shows little effect on the microbial populations of the soils studied. The average microbial population of the oil polluted soil was 9.9 x 103cfu/g, while that without oil-spill was 2.03 x 104cfu/g for bacteria count; average pH value in polluted soil was 5.28 while that of the control was 5.71; average electrical conductivity polluted soil samples was 78.53 while that of the control was 33.7 while moisture content levels of polluted soils was 18.5% and control 44.7%. Bacteriological analysis revealed the presence of bacteria species including; Micrococcus, Pseudomonas, Bacillus and Aerococcus. Statistical analysis of results through the Duncan Multiple range test showed significant differences for microbial populations, electrical conductivity and moisture content of oil polluted soil and the control area (unpolluted) with no significant difference in pH of both polluted and non- polluted soils. It is then concluded that oil spills on the soils of Awoye is detrimental to the microbial population of the soil as it alters negatively the physico-chemical properties of the soil thereby lowering its microbial load and also render the soil and water unfit to support life supporting activities of man.