Abstract: Ethnomedicinal survey and antibacterial study of ethanol and aqueous extracts of Piper guineense and Tetrapluera tetraptera were carried out in Ogbia communities and Microbiology Laboratory Federal University Otuoke respectively. Rapid appraisal method and Use-Value index were applied to fetch information on ethnobotanical use of the two spice plants (commonly used among Ogbia people in Bayelsa State), and their antibacterial activities were tested on Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli and Pseudomonas aeruginosa. Ethnographic data shows that the principal health-care uses of the spices correspond to gastro-intestinal treatment, and fruits were the most important section utilized for managing health disorders. The fruit extracts yielded strong antibacterial activity against the organisms at the treatment regimens (200-12.5mg/ml), the zones of inhibition in both case decreases down concentration gradients. Escherichia coli shows the widest Zone-of-Inhibition in Piper guineense at each concentration regimen. The widest Zone-of-inhibition in Tetrapluera. tetraptera extracts were recorded against S. aureus, and the lowest Minimun-Inhibitory-Concentration, 2.5mg/ml occurred at the assay of Piper guineense against E. coli, and similar trend occurred in Tetrapleura. tetraptera extracts. Also Minimum-Inhibitory-Concentration of 2.5mg/ml and 5mg/ml was recorded on Streptococcus pyogene in ethanol and aqueous extract respectively, justifying the ethnomedicinal uses of the spices among the people.