Poultry meat, as animal-source food, is considered one of the important causes of food-borne illness due to the high possibility of bacterial contamination. The objective of this study was to assess the overall risk of E.coli contamination in broiler food chain in Khartoum North – Sudan. The overall risk of contamination with E.coli was estimated after a qualitative assessment of risk being estimated in poultry farms, slaughterhouse, retailers and consumers respectively. Thirty checklists were taken from poultry farms followed by 30 swab samples collected from one slaughterhouse. The swabs were processed for microbiology and the number of colony forming units (CFU) was then calculated. Additional thirty checklists were collected from retailers, and 50 questionnaires were collected from consumers. High risk at the primary production was detected as a result of high frequencies and impacts of the examined risk factors. The risk of the contamination with E.coli in the slaughterhouse was medium, denoting that the risky event is likely to occur more than once in the next three years. The retailer's risk was high suggesting a great potential of the risky event taking place either during the year in which the study was carried out, or in frequent intervals. The risk added by consumer was high, meaning that the risky event most likely would appear either during the year in which the study was conducted, or in frequent intervals. It is concluded that the overall risk assessment of E. coli contamination in broiler food chain in Khartoum North was high.