The use of domestic waste water for irrigation is a common practice in Nigeria because of the fact that wastes water enhance soil fertility for proper growth and also accommodate a wide spectrum of enteric pathogens and antinutrients that may have a negative impact on human and the environment. This study was conducted to investigate the effect of Antinutritional Factor, Microbial Load and Parasite Quality of Telfairia occidentalis under Controlled Application of Domestic Waste Water. Telfairia occidentalis seeds were planted in 18 pots and 9 pots were irrigated with waste water and the other 9 pot was irrigated with ground water which is use as control till maturity from the month of October to January. Vegetable samples were collected at random with three replications for each test sample after 4 months. The waste water, ground water, soil samples were collected and analyzed for some water and soil physicochemical properties (electrical conductivity, pH and temperature) and soil (pH, total Nitrogen, total phosphorus, organic matter, total organic carbon, and exchangeable cations K+, Mg+ and Na+) using a standard methods. Microbial load such as Total heterotrophic bacteria, E. coli, total coliform, faecal coliform, Staphylococcus aureus, Salmonella and intestinal parasites were determined using standard methods of APHA. Standard titrimeric methods were used to phytate and oxalate contents while Pearson method was used for cyanogenic glycoside, tannin, and alkaloid content. Results showed a significant (p < 0.05) different in water and soil physicochemical properties over the control group. The microbial load in Telfairia occidentalis irrigated with waste water ranged between 8.9×105 to 12.1 × 106 cfu/g while the control site ranged between 1.3×105 to 4.3 × 105 cfu/g. There were significant difference (p<0.05) between the test group and control. The bacterial counts recorded exceeded the recommended levels by WHO and ICMSF, standards (i.e. 10 to 102 coliforms g-1, 1