Abstract: There is a traditional belief that, Nigella sativa seed, commonly known as black seed possess antimicrobial and antioxidant properties. This study was conducted to reveal the antmcrobial and antioxidant property of Nigella sativa seed extract to find out the scientific basis of that belief. For that, powder was made from collected Nigella sativa seed and seed extract was prepared by Soxhlet Method. Qualitative screening for secondary metabolites was performed with Tannins, Saponins, Flavonoids, Alkaloids, Sterol, Terpenoids, Camcf7io Glycosides and Phenols. Quantitative Assay of antioxidant activity was done by DPPH Method. Antibacterial and antifungal activity were done by disc diffusion and tube dilution method. Results showed that N. sativa extracts had highest activity against B. subtilis (20 mm zone). MIC values against all the strains were recorded to be < 100 μg/mL. Antimicrobial activity found to be promising from 100-500 μg/mL, whereas moderate from 500-1000 μg/mL and over 1000 μg/mL the extract showed very weak response. N. sativa presented a significant activity against E. coli and A. alterneria with MIC of 5.85 mg/mL and also against B. subtilis, P. multocida and A. niger with MIC of 23.43 mg/mL. Overall, the antimicrobial activity against fungal strains was low versus bacterial strains.