This study was conducted on 160 poultry farmers in Sokoto state, Nigeria with the use of questionnaire to assess the knowledge, attitude and compliance of poultry workers with preventive measures for poultry diseases in six Local Government Areas of the State. Information obtained from respondents showed that about 82.5% knows about poultry disease with 3.3% through newspaper, 1.5% television, 1.5% friends and 84.9% experience. The most common poultry disease known was Newcastle disease (41%) followed by Gumboro (40%) and coccidiosis (31.9%) among others. Also 60.6% of the respondents have heard about poultry disease outbreak with Avian Influenza having the highest frequency of 63.9%. Only 39.4% had ever experience an outbreak on their farm while 32.5% had never experienced it. Out of all the respondents 46.3% received lectures or training on poultry diseases often and 30% do not. Disease prevention methods employed in both commercial farms and backyard ones includes vaccination (82%), medication (71%), sanitation (43%), disinfection (10.6%), use of foot dip (60.6%), use of farm personnel clothing (59.4%), isolation of sick birds (88.1%). 48.1% of the farmers had their farms free of wild birds (vectors) while the rest had invasions. Twenty-one point nine per cent of the respondents disposed carcasses in refuse dump while 31.9% preferred to bury poultry carcasses. Finally, it was concluded that, the low level of commercial poultry production in the study area might be due to poor practices by the farmers. So, it is recommended that, government should put program about training poultry farmers on biosecurity, disease prevention and adoption of modern husbandry practices suitable for both commercial poultry production system and backyard poultry production system.