Tightness of hamstring muscle is closely linked with movement dysfunction at the lumbar spine, pelvis and lower limbs coupled with low back pain. In the present study, purposively selected 50 collegiate students having asymptomatic hamstring tightness aged 18-26 years from Guru Nanak Dev University, Amritsar, participated in two intervention groups, i.e. Mulligans bent leg raise (BLR) and muscle energy technique (MET). To evaluate the hamstring flexibility, active knee extension test, finger to floor test and sit and reach test of both right and left legs of all the subjects were measured at baseline and after 4th week of intervention. The results indicated significant improvement (p<0.001) in hamstring flexibility in asymptomatic students after the intervention using both Mulligans BLR and MET. After the 4th week intervention, when comparisons were made between these two groups, significant difference (p˂0.05) was found only in active knee extension test of right leg. It could be concluded that both Mulligans BLR and MET were effective in improving the hamstring muscle flexibility in individuals with asymptomatic hamstring muscle tightness. However, MET showed significantly more improvement after 4 weeks of intervention on hamstring flexibility in terms of active knee extension test of right leg as compared to Mulligans BLR technique