Background: Cervical cancer is the second most frequent cancer among women between 15 and 44 years of age and most frequent cancer among women in Nigeria. It is the most preventable cancer known to man. The objectives of the study were to assess the level of awareness, knowledge and determine factors influencing the uptake of cervical cancer screening among women of reproductive age in selected wards of Ede South LGA. Methods: The study was a descriptive cross-sectional study carried out among women of reproductive age in selected wards in Ede South Local Government Area of Ede town, Osun State, Nigeria. Multistage sampling was used as the sampling technique for the study. Data was collected by using a semi structured, self-administered and interviewer guided questionnaire. Results: The mean knowledge score was 16.81+5.12 with 72.8% of the respondents having good knowledge and 26.8% poor knowledge. Though the level of knowledge was high only 11.6% of the respondents had ever had cervical cancer screening done before and 84.5% had never had cervical screening done before. Reported reasons for not screening were no symptoms (33.5%) and no reason (32.9%). 71% were however willing to partake in cervical cancer screening. There were statistically significant associations between practice of cervical cancer screening and age at marriage (p=0.05), religion (p=0.02) respectively. Conclusions: The study revealed a high level of awareness as well as knowledge but a very low level of uptake of cervical cancer screening among women of reproductive age in selected wards of Ede South LGA