The objective of this study was to assessing the nutritional status and its related factors among undergraduate Sudanese University students. As a cross-sectional research, the present study was conducted with the participation of 200 students (111males and 89females) from University of Juba, Sudan . The relevant information was obtained through the anthropometric measurements, a 24-hour diet recall questionnaire were taken for these students. Also, biochemical measurements were taken for 147 students. Majority of the students (61%) were of normal Body Mass Idex (BMI) , 25%, and 14% were underweight, and overweight/obese, respectively. The findings showed that the mean daily intakes of energy, and iron (females only) were less than the RDA(Recommended Dietary Allowances) values. The results showed that 24.49% of the students were anemic, 14.97% iron deficiency anemia, and 3.40% iron deficiency. Hence, a significant relationship was observed between BMI and monthly income, parent's level of education, father occupation, mother occupation, and household size, number of meals, number of snacks, soft drink taken per day, Appetite during exam period, duration daily activities, watching television, anemia (p< 0.05). Regarding BMI categories, some of malnutrition including both underweight and overweight /obese were observed among the students, both these cases are to be considered. Examining the consumed food indicated qualitative and quantitative deficiencies as compared with standard recommendations. The hemoglobin measurement indicated high prevalence of anemia among the students.