Background: This study investigated gender variation in digit ratios of the Bini ethnic group. The study had an analytical cross-sectional design with volunteers age ranging from 18-60 years. For the purpose of this study, an individual was considered to be a Nigerian of a particular ethnic group if the parents and four grandparents are of the same ethnic group. Materials and Methods: Purposive sampling method was used for the study. The selection and collection of required parameters relied on informed consent of volunteers. This was done by giving them a copy of the informed consent letter which was signed and dated. A total of 1,200 subjects (Ikwerre 400, Bini 400, Igbo 400) subjects were recruited for the study. The fingerprints were obtained using print scanner (Hp G3110 Photo scanner). Results and Discussion: The mean digit ratio across the three ethnic groups indicated that the Ikwerre had the following on the left 0.99±0.03; Bini 0.98±0.05 while the Igbos had 0.88±0.02. On the right hand, the mean digit ratio showed that the Ikwerre had 0.98±0.04; Bini 0.98±0.04 while the Igbos had 0.87±0.04. The Ikwerre ethnic group was shown to have a digit ratio closer to the Bini ethnic group than the Igbos and there was no statistical significance (p>0.05) in the comparison of digit ratio between Bini and Ikwerre ethnic groups. There was statistical significance (p<0.05) in the comparison of digit ratio between the Igbos and Ikwerres. Conclusion: The mean of the digit ratios for the Bini people is closer to that of the Ikwerre people than the Igbos. Statistical comparison of the ethnic groups showed that the difference between the Ikwerre and Bini people was not significant meaning that they possibly have ancestral relationship whereas that of the Igbos and Ikwerre people was statistically significant showing that they have a reasonable difference large which ultimately may translate in them not having ancestral relationship.