Divorce cases have been recorded on the high side due to the prevalence of infertility in our society. There is believe that fruits improve fertility. The present study was carried out to evaluate in vivo the effect of date (Phoenix dactylifera) fruit juice on reproductive hormones and antioxidant status in male albino rats. Six (6) rats were divided into two (2) groups of three (3) rats each. (Control and Test). The control was administered distilled water (2ml/kg) while Test was administered 2ml/kg of the fruit juice for twenty-eight (28) days. The result showed that oral administration of the date fruit juice significantly (P˂0.05) increased the level of antioxidant enzymes (SOD and GST) from 2.87±0.15 to 3.87±0.21 and 3.27±0.21 to 4.73±0.15 (Units/mgprotein) respectively. The level of Testosterone also increased significantly (P<0.05) from 0.57±0.03 to 0.94±0.08 (ng/ml), while the activity of FSH and LH hormones increased nonsignificantly (P˃0.05) from 20.23±0.86 to 21.87±0.65 and 11.00±0.34 to 11.57±0.72(miu/ml) respectively. There was a significant weight gain in the testes administered 2 ml/kg date fruit juice as compared to the control. Our result implies that date fruit juice had a significant effect on the testis and increased the level of testicular hormones and antioxidant enzymes. This justify the conclusion that the juice may improve fertility.