The present study revealed the presence of some bioactive ingredients such as; Saponin, Flavonoids, Tannins, Alkaloids, Phenols, and Phyto Sterosl in both plants only tannin compound was absent in Psidium guajava extracts as slated in table 2. The antibacterial activity of ethanolic and aqueous extracts of leaves of Citrus aurantifolia and Psidium guajava were analyzed using standard procedure of Various concentrations of ethnolic and aqueous extracts viz; 50,40,30,20,10mg/ml respectively, as well as tested against the bacterial isolates.The bacterial isolates include ; Escherichia coli and Pseudomonas aeruginosa were both gram negetive bacteria. The highest zone of inhibitions of 20.0mm at 50mg/ml on E. coli and 15.0mm on P. aeruginosa at 50mg/ml were detected on aqueous leaves extracts, whereas, the ethanolic extract of C. aurantifolia showed zone of inhibitions on E. coli at 30, 40, 50mg/ml and no zone of inhibitions shown on P.aeruginosa except at 30mg/ml. Moreover, the aqueous extracts of P. guajava also showed the zone of inhibitions of 10mg/ml and 30mg/ml on E. coli, whilst on P. aeruginosa there was no zone of inhibitions, as same as on ethanolic extract of P. guajava at 10mg/ml on E. coli has no zone of inhibitions. The zones of inhibitions on both extracts (ethanolic and aqueous) were significant in only 1% to E .coli whereas, in P. aeruginosa there was no significant difference at 5%, as showed in table 4,5 and 6 as well as presented in the charts in elaborate.