Prunus cerasus leaves (PCL) and Chamaemelum nobile stalks (CNS) aqueous extracts have antimicrobial, antioxidant and pharmacological properties. This study aimed to assess PCL and CNS antibacterial effects on both pathogenic strains (Escherichia coli and Staphylococcus aureus). Extraction methods were used for this experiments including maceration, decoction and infusion of aerial plant parts (leaves and stalks). Different concentrations of the aerial plant part aqueous extracts were tested and have shown their antimicrobial activity by spreading method from a well on Mueller-Hinton agar.PCL aqueous extract was active on both bacteria strains with different minimal inhibitory concentrations (MIC) as follow: 125 < MIC < 375 ×106 μg / ml for S. aureus and 250 < MIC < 500 ×106 μg / ml for E. coli. The strain of E. coli was resistant to both antibiotics used namely AMC and E15 whereas S. aureus was resistant to both antibiotics OX and E15. The antimicrobial activity of PCL was greater than that of CNS aqueous extract with a broader antimicrobial spectrum at lower concentrations. Prunus cerasus and Chamaemelum nobile, as aromatic plants, could be used in alternative medicine and having striking features to prevent urinary tract infections.