“EAS Journal of Biotechnology and Genetics” ISSN 2663-189X (Print) and ISSN 2663-7286(Online) is monthly, peer reviewed, open access Academic and Research Journal which publishes Full Length Original Research Articles, Review Articles, Mini Reviews, Essays, Short Communications, Case Studies, editorial comments etc in all the fields of Biotechnology and Genetics.
Scope of Journal
The aim of the “EAS Journal of Biotechnology and Genetics” is to promote excellence by providing a venue for academicians, Researchers and students to publish significant empirical and conceptual findings in all the fields of Biotechnology and Genetics and their applied fields and share globally.
“EAS Journal of Biotechnology and Genetics” publishes in all the fields of Biotechnology and Genetics like Biophysics, Biochemistry, Bioinformatics, Bioenergy, Biosafety, Biosecurity, Bioethics, Medical Biotechnology, Pharmaceutical biotechnology, agricultural biotechnology, Molecular genetics, Evoluationary genetics, Developmental genetics, Heredity genetics, Behavioural genetics, Genetic analysis, Gene regulation, Gene expression profiling, Genetic variation, Epigenetics, Gene therapies, Cellular genetics and molecular biology, Population genetics, Quantitative and computational genetics, Microbial genetics, Genetics in medical field, Signal transduction, Genome and systems biology, genetic disorders, Computational molecular biology, rDNA, CRISPR, and all other genetic and molecular biology techniques etc.
Editor in Chief:
Dr. T. Selvankumar
Professor & Head PG & Research Dept. of Biotechnology (DST-FIST Sponsored) Mahendra Arts & Science College (Autonomous) Kalippatti, Namakkal- 637 501, Tamil Nadu, India Email: email@example.com
Mary Esien Kooffreh
Dept of Genetics and Biotechnology, University of Calabar, P.M.B 1115 Calabar, Nigeria Email: firstname.lastname@example.org
Dr. Benard Chemwei, PhD
Researcher and Academic Kabarak University Private Bag 20157, Kenya Email: email@example.com
Dr Nouar Hind
Department of Ecology and Plant Biology, Faculty of Nature and Life Sciences, Ferhat ABBAS University, Setif -1, 19000, Algeria Email: firstname.lastname@example.org
Ms.c, Anatomy and Cell Biology, Shahid Sadoughi University of Medical Science and Health Services, Yazd, Iran. Email: email@example.com
Asma Mami Maazoun
Post-doctoral researcher, Laboratory of Biotechnology Applied to Agriculture (LBAA); National Agricultural Research Institute of Tunisia, Avenue rue Mokhtar Attia 70, 1000 Tunis, Tunisia Tel: (00216) 29883165 E-mail: firstname.lastname@example.org
Nduche, Michael Ugochukwu
Department of Plant Science and Biotechnology, Michael Okpara University of Agriculture Umudike Abia State, Nigeria Email: email@example.com
Ordu University, Department of Molecular Biology and Genetics, Faculty of Science and Letters, 52200 Ordu-Turkey Email: firstname.lastname@example.org
Datura stramonium contains allelochemicals which have allelopathic properties that have the potential to inhibit or stimulate vegetative plant growth. Aqueous leaf extract of D. stramonium at 25%, 50% and 100% concentrations were applied to determine their effects on plant height, number of leaves per plant, leaf length, root length and internode length of two maize varieties (Sammaz-39 and Oba super-06) under field conditions. Distilled water (0%) served as control.The treated maize seeds were planted in the experimental field and the plots were laid as randomizedcomplete block design (RCBD) replicated three times.Results on the effects of D. stramonium leaf extract were determined for each treatment concentration. Results showed that D. stramonium leaf extract significantly increased plant height of Sammaz-39 at a concentration of 50% at 10WAP and also of Oba super-06 variety at a concentration of 50% at 8 and 10WAP. Number of leaves per plant was significantly decreased at 10WAP in Sammaz-39 at 100% concentration while Oba super-06 variety showed a significant decrease in number of leaves per plant at 50% concentration at 8WAP. The highest leaf length was observed at 25% concentration of D. stramonium leaf extract in Sammaz-39 at 8WAP and at 25% concentration for Oba super-06 at 2, 4, 6 and 8WAP. But at 10WAP, highest leaf length was observed in the control (0%). Root length was significantly increased in treated seeds of the two varieties; highest root length was observed at 50% concentration in both Sammaz-39 and Oba super-06. Internode length on the contrary, was significantly decreased at high concentration of 100% in Sammaz-39 while no significant difference was observed in the internode length of Oba super-06 variety. This study reveals that high concentration (100%) of D. stramonium leaf extract has inhibitory effects on number of leaves per plant, leaf length and internode length while lower concentrations (25% and 50%) has stimulatory effects on vegetati
Background: The cytochrome c, somatic (CYCS) gene encodes a small haeme protein that functions as a central component of the electron transport chain in mitochondria. Most important health condition related to mutations in the coding region of this gene was Thrombocytopenia 4 and affect apoptosis. Aims: This study aimed to investigate the effect of non-synonymous SNPs (ns SNPs) of CYCS gene in protein function and structure using different computational software. Material and Methods: Different nsSNPs and protein related sequences were obtained from NCBI and ExPASY database (2020). Deleterious and damaging effect of SNPs were analyzed using SIFT, Provean, Polyphen-2 and SNPs & GO software. Protein stability was investigated using I-Mutant and MUpro software. The interaction of CYCS with other genes was studied using GeneMANIA software. The structural and functional impact of point mutations was predicted using Project Hope software. Results: A total of 100 Single Nucleotide Polymorphisms were retrieved from National Center of Biotechnology Information (NCBI). From these 68 were in the 3`UTR, 28 in the 5`UTR and 14 in the coding region (nsSNP). Only these 14 were selected for further investigations. Six nsSNPs were found to be deleterious while 8 were tolerated by SIFT. Using Provean 11 nsSNPs were found to be deleterious while 3 were Neutral. For PolyPhen-2, 5 nsSNPs were observed to be damaging while 9 were benign. Using PHD and SNPs&GO 9 and 6 nsSNPs were found to be disease related for the two software respectively. The change in the chemical nature of amino acid and how it affects the protein structure was analyzed using Project Hope. Conclusion: Six highly deleterious, damaging and disease related nsSNPs (rs17851278, rs11548796, rs121918552, rs11548820, rs11548818 and rs11548778) were detected at CYCS gene. These nsSNPs can be considered as candidate nsSNPs for diagnosis of Thrombocytopenia 4 and normal apoptosis.
Disorders of sex development (DSD) are defined as congenital conditions associated with atypical development of chromosomal, gonadal or phenotypical sex. Majority of 46XY DSD patients present clinical characteristics of primary amenorrhea. However few cases with secondary amenorrhea are reported in the literature. It was suggested that such patients might have an estrogen secretion by a tumoral lesion. In other cases, it could be a misdiagnosed 46,XY DSD following bone marrow transplantation. We report here the third case of misdiagnosed 46,XY DSD following bone marrow transplantation. Our patient is a 15-year old girl referred to our department of endocrinology for secondary amenorrhea. She had been diagnosed with lymphoblastic Leukemia at the age of 13. She received chemotherapy and total body irradiation after which she had bone marrow transplantation. The hormonal profile confirmed the primary ovarian insufficiency. Chromosomal analysis performed on peripheral blood lymphocytes showed a 46,XY karyotype consisting with a diagnosis of 46, XYDSD. However, regarding her past medical history, we complete with karyotype on skin fibroblasts that showed a normal female karyotype. Thus we conclude that our patient had a misdiagnosed 46,XYDSD, and that her secondary amenorrhea is caused by chemotherapy and radiotherapy. The fertility prognosis is considered poor in that case. Although the need for fertility preservation has to be weighed against morbidity and mortality associated with cancer, our findings highlight the importance of fertility preservation by oocyte and/or embryo cryopreservation for such patients.
Enteropathogenic Escherichia coli is one of the most important pathogens causing severe and persistent watery diarrhoea, abdominal pain, fever, vomiting and often lead to death in humans. Diarrhoeic faecal samples were collected from 210 children (aged < 5 years) in three different hospitals which are Federal Medical centre Idi-Aba Abeokuta, Otunba Tunwase Padectrics Centre, Ijebu Ode and Olabisi Onabanjo Teaching hospital Sagamu after questionaires were administered. The stool samples were cultured for bacteria on Eosin methylene blue agar, MacConkey agar, and Salmonella-Shigella agar. Escherichia coli were identified using biochemical characterization. Enteropathogenic Escherichia coli were serotyped by slide agglutination test using specific Enteropathogenic Escherichia coli monovalent antisera (EPEC O111, O126, O86, O55, O128 and O26). Antibiotic sensitivity was carried out using disc diffusion method. Plasmids from multi-drug resistant Enteropathogenic Escherichia coli were extracted by Alkali-lysis method and electrophoresed. Genomic DNA were extracted from Enteropathogenic Escherichia coli and subjected to multiplex Polymerase Chain Reaction for detection of effacing and attaching (eae), bundle-forming pili (bfp) and enteropathogenic Escherichia coli adherence factor (eaef) genes. The strains that exhibited multiple drug resistant were 21(18.8%) with plasmids detected in 12(57.1%) of the 21 multi-drug resistance Escherichia coli. Twenty-one of the Enteropathogenic Escherichia coli strains possessed effacing and attaching genes, 6 possessed bfp while 18 possessed eaef. Typical Enteropathogenic Escherichia coli strains were more prevalent (tEPEC) with (71.4%) than atypical (aEPEC) (28.6%). The molecular characterization of the Enteropathogenic Escherichia coli among children in this study, revealed typical Enteropathogenic Escherichia coli (tEPEC) as the dominant strain in Ogun state.
Jiménez Leiva Alejandra, Madrigal Elizondo José Antonio, Murillo Muñoz Melissa, Rojas Lezama Marilyn, Villegas Campos Mauricio, Mora Román Juan José
East African Scholars J Biotechnol Genet, 2020; 2(1):1-11.
DOI : 10.36349/EASJBG.2020.v02i01.001
Cancer is a disease that has positioned itself as the second cause of death globally. However, there is a deficiency in diagnostic tools and treatment. Traditionally, biopsy involves tissues extraction to be examined. But, it is an invasive procedure and can lead to complications in patients. Liquid biopsy has been developed as a non-invasive technique, with lower cost and allowing a better diagnosis. As a complement, new-generation sequencing (NGS) allows information sequencing and analysis from DNA. The combined use of these tools can result in identification of genetic aberrations in cancer, improving of patients´ prognosis, and use of personalized therapies when studying an individual´s genomics, epigenomics, and proteomics profile. Despite these advances, there are elements to improve procedures sensitivity, a better understanding of biochemistry and immunological response in tumorigenesis processes, and integration of this data through computational strategies for the development of tumor profiles
Medicinal plants have been used as remedies for human diseases because they contain components of therapeutic value. Plants serve as the greatest source to obtain new antimicrobials. An increasing interest in herbal remedies has been observed in several parts of the world. Tridax procumbens Linn. commonly known as ‘coat button’ is a weed and a pest plant. Previous researches have shown that the plant has antimicrobial activities and pharmacological effects. This study aimed at evaluating the phytochemical compounds and antibacterial activities of the leaf extract of Tridax procumbens. Leaf ethanol and water extracts were used in this study. The test organisms were Staphylococcus aureus and Escherichia coli. The antibacterial assay was performed by disc diffusion method. Treatment extract concentrations were 250mg/ml, 200mg/ml, 100mg/ml and 50mg/ml and distilled water (negative control) and an antibiotic Amphicloxa (positive control). Each treatment was replicated three times. The leaf extract was tested for the presence of tannins, saponins, flavonoids, terpenoids, alkaloids and glycosides. The plates were examined for zones of inhibitions. Data collected was subjected to analysis of variance. Treatments were separated and compared using LSD at P < 0.05. Both ethanol and water extracts were biologically active against the two microbes. Ethanol extract showed greater inhibition (9.29 mm) in comparison to water extract (7.19 mm). Escherichia coli showed greater susceptibility as compared to Staphycoccus aureus. The phytochemical screening of the leaf extract of Tridax procumbens revealed the presence of tannins, alkaloids, saponins, flavonoids, terpenoids and glycosides. It is possible that the growth inhibition observed in the study occurred due to the presence of these different chemical compounds. From these findings, ethanolic and water extracts of the leaf of Tridax procumbens Linn. showed a good potential as a source of new drug for treating infecti
This study was initiated to generate information about genetic variability, heritability and genetic advance of
yield components in Citrullus mucosospermus. In this way, four inbred lines of C. mucosospermus were evaluated at
Kononfla city in Western Côte d’Ivoire, using a randomized complete block design with three repetitions. The results of
discriminant analysis indicated significant differences between four C. mucosospermus inbred lines what are Bebu,
Wlêwlê small seeds 1, Wlêwlê small seeds 2 and Wlêwlê small seeds 3. Mahalanobis distances varied significantly from
8.44 to 629.50 between theses inbred lines. All investigated traits recorded high genotypic and phenotypic coefficients of
variation values except fruit maturity period and fruit diameter where, low values were recorded. Studied morphological
traits exhibited high heritability (H²> 80%) coupled with moderate to high genetic advance as percentage of mean.
Stepwise regression analysis revealed mass of fresh seeds per fruit followed by mass of fruit, seed width, seed length and
fruit maturity as traits contributing for linear increase in mass of dry seeds. These traits could be considered as key
criteria for selecting high yielding line in C. mucosospermus breeding program at Côte d’Ivoire.
Dr. T. Selvankumar Chief Editor EAS Journal of Biotechnology and Genetics
Dr BOUCENNA Mounir Chief Editor EAS Journal of Veterinary Medical Science
Dr. James Kay, PhD Chief Editor EAS Journal of Psychology and Behavioural Sciences
Dr. Rejeesh Menon Chief Editor EAS Journal of Medicine and Surgery
Dr. S. Jayachandran Chief Editor EAS Journal of Dentistry and Oral Medicine
Dr. Hamid Osman Hamid Chief Editor EAS Journal of Radiology and Imaging Technology
Dr. Md. Habibur Rahman Chief Editor EAS Journal of Pharmacy and Pharmacology
Dr. Benard Chemwei, PhD Chief Editor East African Scholars Multidisciplinary Bulletin
Prof. Dr. Nazir Ahmad Suhail Chief Editor East African Scholars Journal of Engineering and Computer Sciences
NFI Joseph Lon Chief Editor EAS Journal of Humanities and Cultural Studies
Dr. Muslim A. Djalil Executive Editor Fakultas Ekonomi & Business, Universitas Syiah Kuala, Kopelma Darussalam, Banda Aceh,23111 Indonesia, email:email@example.com
EAS Publisher (East African Scholars Publisher) is an international scholar’s publisher for open access scientific journals in both print and online publishing from Kenya. Its aim is to provide scholars ... Read More Here
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